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this was particularly evident for patients treated with once-weekly GLP-1 RAs at 12 months post-index, where a mean weight loss of 2.8 kg (95% CI 3.6, 2.1) was reported for adherent patients, whereas poor adherence was associated with a weight gain of 0.2 kg ( 0.5, 0.9) (Supplemental Fig

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Yeast two-hybrid and yeast one-hybrid assays To conduct yeast two-hybrid (Y2H) screening, the full-length CDS of the relevant genes were cloned into the bait vector pGBKT7 and the prey vector pGADT7, respectively